Generator

Part:BBa_K223045:Design

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-10-17)


RBS- RALDH II- TT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 1579
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal SpeI site found at 1580
    Illegal PstI site found at 1594
    Illegal NotI site found at 27
    Illegal NotI site found at 1587
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal BglII site found at 137
    Illegal BglII site found at 1271
    Illegal XhoI site found at 118
    Illegal XhoI site found at 1174
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 1580
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 21
    Illegal XbaI site found at 36
    Illegal SpeI site found at 1580
    Illegal PstI site found at 1594
    Illegal NgoMIV site found at 651
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 989


Design Notes

n/a


Source

Stanford iGEM 2009

References